PHCBI PRIMESURFACE Ultra Low Attachment 3D Cell Culture Equipment

What is the Concept of Cell Culture?

Cell culture is the process of growing cells under controlled conditions. In practice, the term cell culture is used for the cultivation of cells derived from multicellular eukaryotes, especially animal cells.

What is Cell Culture Used For?

Studies conducted with cell cultures constitute an important part of popular research topics today. For example, cell cultures can be used to determine the effects of a certain substance in various pathological conditions such as cancer, or to study the functions of a substance produced in a cell or tissue.

What is the Technique of Cell Culture?

The cell culture method is a biotechnological technique that refers to the cultivation of cells under laboratory conditions and in special environments, outside of their natural habitat.

How is Cell Culture Performed?

Cells are seeded into plastic culture vessels and cultured in growth medium. After a few days, the cells migrate from the tissue explant to the surface of the culture vessel, where they divide and grow. This results in the formation of a suspension. Later, these cells are transferred into another culture vessel containing growth medium.

What Should the Cell Culture Environment Be Like?

The cell culture cabinet should be large enough for one person to use, easy to clean inside and out, and sufficiently illuminated. The working area must always be clean and organized. Materials in the cell culture cabinet should be cleaned with 70% ethanol.

How is Cell Culture Classified?

Primary culture, cell lines, suspension culture, adherent culture, cancer cells, and literature studies.

What is a Cell Culture Laboratory?

In a cell culture laboratory, molecular mechanisms of various cells cultured in vitro are studied. Additionally, cellular modeling of diseases is carried out to investigate their molecular basis.

Advantages of Cell and Tissue Cultures

Tissue culture is the cultivation of tissues and cells outside the organism (ex vivo) using liquid, semi-solid, or solid media. Tissue culture can be used interchangeably with cell culture, but it may also refer to the in vitro cultivation of a piece of tissue taken from a living organism. It reduces the need to sacrifice large numbers of animals. Cell lines can be easily transported. Since quarantine is not an issue, experiments and applications can be carried out under the same conditions even in distant countries.

What are the Applications of Cell Culture?

  • Stem cell research
  • IVF and infertility treatments
  • Observation of cellular metabolic activities
  • Drug development
  • Vaccine production
  • Conducting experiments that are difficult to perform in living organisms
  • Conservation of endangered species
  • Cultivation of species that are difficult to reproduce

Advantages of Cell and Tissue Cultures

The growth medium controls the culture’s pH and protects it against changes. Its balance is delicate, and changes in atmospheric CO2 can alter the pH of the medium. For this reason, when using CO2-bicarbonate–based buffered media, the use of an external CO2 source is important.
See CO2 Incubator PHCBI (Panasonic/SANYO)

Transition from 2D to 3D Culture Techniques

Two-dimensional (2D) cell culture is a valuable method for cell-based research, but it can provide unpredictable and misleading data regarding in vivo responses. The new paradigm of 3D cell culture has become popular. The transition from 2D to 3D culture techniques is an important step toward physiologically more relevant tissue models. 3D cell cultures offer different techniques for different purposes, allowing users to select the appropriate model for their needs. 3D cell culture systems are used in stem cell studies, drug discovery, cancer research, gene and protein expression studies, and many more. With the emergence of 3D cultures, in vitro studies now offer superior structures for investigating complex interactions that were not possible with 2D cultures.

What is 3D Cell Culture?

3D cell culture is an artificially created environment that allows biological cells to grow in three dimensions. Unlike 2D environments, a 3D cell culture provides insight into how cells grow in vivo while being maintained in vitro.
Three-dimensional (3D) cell culture techniques are an innovation developed to overcome the limitations of adherent cell culture. Various methods have been developed to produce in vitro tissue analogs. These methods may require expensive and complex specialized equipment and are often limited by compatibility with specific cell types. However, the 3D cell culture technique describes a rapid, flexible, and reproducible protocol that allows cells to aggregate into multiple 3D spheroids of suitable size, compatible with the growth of tumor and normal cell lines. The generated 3D spheroids can be collected for use in a wide range of applications, including studies of cell signaling or gene expression, drug screening, or cellular processes such as tumor invasion and migration. The protocol has also been adapted to generate clonal cell spheroids, and to assess anchorage-independent growth and anoikis resistance. In stem cell research, it offers a cost-effective, practical, reproducible, and scalable platform for broad research opportunities.

Which Diseases Can Be Treated with Stem Cells?

  • Bone marrow cancers
  • Lymphoma
  • Hodgkin’s lymphoma
  • Leukemia
  • Anemia
  • Thalassemia
  • Organ cancers
  • Plasma cell disorders
  • Bone marrow failure
  • Multiple myeloma
  • Hereditary and congenital blood diseases
  • Diseases caused by immune deficiency
  • Hereditary metabolic diseases

What are Cell Culture, Tissue Culture, and Artificial Organs?

These are mechanical materials or engineered tissues designed to replace organs that have lost or are losing their function, usually vital ones, in order to restore part or all of their functions.

What is an Organoid?

An organoid is a miniature and simplified version of an organ produced in vitro from stem cells treated with various differentiation factors.

How is an Organoid Produced?

For example, researchers at Columbia University Medical Center produced a mini-lung in 3D structure from human pluripotent stem cells that mimicked lung functions.

Organoid Model

Organoids are three-dimensional mini tissue cultures produced in vitro from stem cells to replicate specific organs. These mini-cultures can mimic the unique characteristics of an organ or exhibit organ-specific behaviors, such as producing certain cell types. This method leverages the unlimited self-renewal and differentiation capacity of stem cells.

PHCBI - Primesurface 3D Ultra Low Attachment Cell Culture Plates

PHCBI - Primesurface 3D Ultra Low Attachment Cell Culture Plates
They provide high-quality three-dimensional cell culture platforms with various well shapes to support the spheroid culture of your specific cell type. These are ultra-low attachment (ULA) dishes and plates that support the scaffold-free self-assembly of spheroidal formations. Plates are pre-coated with a unique ultra-hydrophilic polymer that ensures uniform spheroid/EB formation, preventing cell adhesion, cytotoxicity, and material degradation. ULA plates have high optical clarity, making them highly suitable for bright-field imaging and confocal microscopy. In addition to the commonly used 96-well U-bottom plate, V-bottom and M-bottom 96-well plates are also available. For high-throughput screening (HTS) needs, 384-well plates are available in both transparent and white formats.
The PrimeSurface series is coated with a unique ultra-hydrophilic polymer covalently bound to the plastic surface, effectively preventing cell adhesion without cytotoxicity or material degradation. Superior coating technologies and production processes ensure smooth surfaces and uniform spheroid/EB formation for clear cell imaging.

Advantages

  • Non-adhesive surface for cells to facilitate natural spheroid formation
  • Uniform single spheroid/EB formation per well
  • Simultaneous spheroid formation and analysis in the same plate
  • Various well-bottom shapes: U-bottom, M-bottom, and V-bottom in 96-well format
  • High optical clarity plates for imaging
  • Stable, non-cytotoxic, and cell-repellent surface
  • Easy to use, compatible with liquid handling systems
  • 384-well format for high-throughput assays
  • Compatible with bright-field and fluorescence imaging systems
  • White plates compatible with luminescence assays

96 Slit-Well Plate

With the introduction of the PrimeSurface 96 Slit-Well Plate, medium exchange for 96 wells can be performed efficiently by one-step dispensing or aspiration of all wells simultaneously. This product can reduce pipetting time by over 80% while minimizing the risk of spheroid damage.

Advantages

  • Generates uniform spheroids
  • Medium exchange without disrupting spheroid formation
  • Minimizes medium exchange time by delivering culture medium to all 96 wells simultaneously
  • Provides up to 1.5 times more medium, fewer medium changes, and higher yield compared to conventional plates
96 Slit-Well Plate
Cat. No. Microplates Product Name Number Of Wells Color Well Bottom Maximum Well Volume Package
MS-9096SZ PrimeSurface 96 Slit-Well Plate 96 Clear Spindle 0.3 ml Individually packed, 20 plates/case
96 Slit-Well Plate
PrimeSurface ultra low attachment (ULA) cell culture dishes are also available.
There are 3 types of PrimeSurface Dishes in total:
  • PrimeSurface Dish 35 mm
  • PrimeSurface Dish 60 mm
  • PrimeSurface Dish 90 mm
Color Clear
Well Type Flat (9 cm2)
Package (radiation sterilized) 5 dishes/pack, 50 dishes/box